evaluation of rhizoctoni solani control in cotton by pseudomonas fluorescens isolates

نویسندگان

فاطمه زهرا عبداللهی پور

کارشناسی ارشد بیماری شناسی گیاهی، گروه گیاه پزشکی، دانشگاه شهید مدنی آذربایجان اکبر شیرزاد

استادیار، گروه گیاه پزشکی، دانشگاه شهید مدنی آذربایجان حمید محمدی

استادیار، گروه زراعت و اصلاح نباتات، دانشگاه شهید مدنی آذربایجان

چکیده

plant growth-promoting rhizobacteria (pgpr) is an alternative to chemical pesticides against harmful microorganisms especially soil-borne diseases. rhizoctonia solani is one of the most important soil-borne fungal pathogens that causes serious damages to important crops. in this study, to screen successful pseudomonas bacterial isolates, the inhibition of pathogen growth was evaluated in-vitro. moreover, isolates were screened based on the ability to auxin and acc-deaminase production, phosphorus solubilization, siderophore production and possession of phld gene. from isolates studied for the extent of inhibition, 20 isolates were selected for further studies, among them, the isolates 33, 139 and 137 showed the highest rate of p solubility and ability to produce auxin. there was a correlation between the phosphorus solubility and ph reduction. six isolates were selected to evaluate their capability for acc-deaminase enzyme production based on the higher auxin production and better in-vitro inhibitory effect on the growth of rhizoctoniasolani. all of the six strains appeared to have produce acc-deaminase enzyme. phld gene that plays a key role in 2,4-diacetylphloroglucinol antibiotic biosynthesis pathway, was detected in the isolates 153, 139, 137, cha0 and 58a. according to the characteristics, five isolates were selected for greenhouse experiments. in greenhouse experiments, all five isolates controlled disease significantly, and among them, the 137 isolate showed the highest control level

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Control of Rhizoctonia solani on Cotton Seedlings with Pseudomonas fluorescens and With an Antibiotic Produced by the Bacterium

HOWELL, C. R., and R. D. STIPANOVIC. 1979. Control of Rhizoctonia solani on cotton seedlings with Pseudomonasfluorescens and with an antibiotic produced by the bacterium. Phytopathology 69:480-482. A strain of Pseudomonasfluorescens antagonistic to Rhizoctonia solani Alternaria sp., and Verticillium dahliae. A Fusarium sp. was only partially was isolated from the rhizosphere of cotton seedlings...

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عنوان ژورنال:
کنترل بیولوژیک آفات و بیماری های گیاهی

جلد ۴، شماره ۱، صفحات ۲۳-۳۶

کلمات کلیدی
[ ' p l a n t g r o w t h ' , ' p r o m o t i n g r h i z o b a c t e r i a ( p g p r ) i s a n a l t e r n a t i v e t o c h e m i c a l p e s t i c i d e s a g a i n s t h a r m f u l m i c r o o r g a n i s m s e s p e c i a l l y s o i l ' , ' b o r n e d i s e a s e s . r h i z o c t o n i a s o l a n i i s o n e o f t h e m o s t i m p o r t a n t s o i l ' , ' b o r n e f u n g a l p a t h o g e n s t h a t c a u s e s s e r i o u s d a m a g e s t o i m p o r t a n t c r o p s . i n t h i s s t u d y ' , ' t o s c r e e n s u c c e s s f u l p s e u d o m o n a s b a c t e r i a l i s o l a t e s ' , ' t h e i n h i b i t i o n o f p a t h o g e n g r o w t h w a s e v a l u a t e d i n ' , ' v i t r o . m o r e o v e r ' , ' i s o l a t e s w e r e s c r e e n e d b a s e d o n t h e a b i l i t y t o a u x i n a n d a c c ' , ' d e a m i n a s e p r o d u c t i o n ' , ' p h o s p h o r u s s o l u b i l i z a t i o n ' , ' s i d e r o p h o r e p r o d u c t i o n a n d p o s s e s s i o n o f p h l d g e n e . f r o m i s o l a t e s s t u d i e d f o r t h e e x t e n t o f i n h i b i t i o n ' , ' 2 0 i s o l a t e s w e r e s e l e c t e d f o r f u r t h e r s t u d i e s ' , ' a m o n g t h e m ' , ' t h e i s o l a t e s 3 3 ' , ' 1 3 9 a n d 1 3 7 s h o w e d t h e h i g h e s t r a t e o f p s o l u b i l i t y a n d a b i l i t y t o p r o d u c e a u x i n . t h e r e w a s a c o r r e l a t i o n b e t w e e n t h e p h o s p h o r u s s o l u b i l i t y a n d p h r e d u c t i o n . s i x i s o l a t e s w e r e s e l e c t e d t o e v a l u a t e t h e i r c a p a b i l i t y f o r a c c ' , ' d e a m i n a s e e n z y m e p r o d u c t i o n b a s e d o n t h e h i g h e r a u x i n p r o d u c t i o n a n d b e t t e r i n ' , ' v i t r o i n h i b i t o r y e f f e c t o n t h e g r o w t h o f r h i z o c t o n i a s o l a n i . a l l o f t h e s i x s t r a i n s a p p e a r e d t o h a v e p r o d u c e a c c ' , ' d e a m i n a s e e n z y m e . p h l d g e n e t h a t p l a y s a k e y r o l e i n 2 ' , 4 , ' d i a c e t y l p h l o r o g l u c i n o l a n t i b i o t i c b i o s y n t h e s i s p a t h w a y ' , ' w a s d e t e c t e d i n t h e i s o l a t e s 1 5 3 ' , 1 3 9 , 1 3 7 , ' c h a 0 a n d 5 8 a . a c c o r d i n g t o t h e c h a r a c t e r i s t i c s ' , ' f i v e i s o l a t e s w e r e s e l e c t e d f o r g r e e n h o u s e e x p e r i m e n t s . i n g r e e n h o u s e e x p e r i m e n t s ' , ' a l l f i v e i s o l a t e s c o n t r o l l e d d i s e a s e s i g n i f i c a n t l y ' , ' a n d a m o n g t h e m ' , ' t h e 1 3 7 i s o l a t e s h o w e d t h e h i g h e s t c o n t r o l l e v e l ' ]

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